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Biacore carboxymethyl dextran cm 5 sensor chip
Carboxymethyl Dextran Cm 5 Sensor Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cm-5+sensor+chips/biosensor+chips+dextran/pmc13039201-132-17-29
Average 86 stars, based on 1 article reviews
carboxymethyl dextran cm 5 sensor chip - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Molecular design of N-linked tetravalent glycosides bearing N-acetylglucosamine, N,N'-diacetylchitobiose and N-acetyllactosamine: Analysis of cross-linking activities with WGA and ECA lectins.
Article Snippet: CM-5 sensor chips and the amine coupling kit, containing N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-hydrochloride (EDC) and ethanolamine-hydrochloride, were purchased from Biacore AB (Uppsala, Sweden).

Article Title: A radio-isotope label-free ?-bungarotoxin binding assay using Biacore sensor chip technology for real-time analysis
Article Snippet: The ligand, α-bgtx, that we directly immobilized onto BIAcore CM-5 sensor chips, is a 74-amino acid α-neurotoxin isolated from the venom of the Taiwanese banded krait, Bungarus multicinctus [ 5 ].

Article Title: T cell receptors
Article Snippet: BIAcore Method Biotinylated peptide-HLA monomers were immobilized on to streptavidin-coupled CM-5 sensor chips.

Article Title: Engineering of Single Ig Superfamily Domain of Intercellular Adhesion Molecule 1 (ICAM-1) for Native Fold and Function
Article Snippet: Surface Plasmon Resonance Surface plasmon resonance (SPR) experiments were carried out using a Biacore 2000 instrument with CM-5 sensor chips using N -hydroxysuccinimide/ N -ethyl- N ′(dimethyl-aminopropyl) carbodiimide chemistry for the immobilization.

SPR Assay:

Article Title: Methods and compositions for modulating tumor cell activity
Article Snippet: .. An extra cysteine was added to the C-terminus of the peptide to facilitate oriented coupling on the surface of the CM-5 sensor chips that were used for screening of the rabbit antisera by surface plasmon resonance (SPR, Biacore.TM. ..

Article Title: Engineering of Single Ig Superfamily Domain of Intercellular Adhesion Molecule 1 (ICAM-1) for Native Fold and Function
Article Snippet: After the refolding step, the refolding solution was filtered through a 0.22- m filter, concentrated, and subjected to gel filtration chromatography using Superdex 75 column connected to AKTA Purifier (GE Healthcare). .. Surface Plasmon Resonance—Surface plasmon resonance (SPR) experiments were carried out using a Biacore 2000 instrument with CM-5 sensor chips using N-hydroxysuccinimide/N-ethyl-N (dimethyl-aminopropyl) carbodiimide chemistry for the immobilization. .. To achieve functional coupling of the HA I domain, we introduced a cysteine residue to the N terminusthatwassubsequentlyincubatedwithmaleimide-polyethylene oxide-biotin (Pierce) for biotinylation following the manufacturers protocol.

Polymer:

Article Title: Rational Design and Characterization of Trispecific Antibodies Targeting the HIV-1 Receptor and Envelope Glycoprotein.
Article Snippet: .. HIV-1 proteins were amine coupled to a carboxylated dextran polymer matrix, which was coated on CM-5 sensor chips for use in the BIAcore 3000. .. The carboxyl groups on the chips were activated with 35 μL of a 1:1 (v/v) solution of N-hydroxysuccinimide and Nethyl-N-(3-diethylaminopropyl) carbodiimide for 7 min. HIV-1 proteins at a concentration of 50 μg/mL were prepared using 20 mM sodium acetate as a diluent and each coated onto one cell, whereas the other cell was left blank as a reference and blocked with 1 M ethanolamine HCl, pH 8.5 (GE Healthcare, Chicago, United States) as a control.



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KEY RESOURCES TABLE

Journal: Structure (London, England : 1993)

Article Title: Structural adaptation in its orphan domain engenders betaglycan with an alternate mode of growth factor binding relative to endoglin

doi: 10.1016/j.str.2019.06.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CM-5 SPR sensor chip , GE Lifesciences , Cat# 29149604.

Techniques: Recombinant, Software